Baculovirus Expression of BmAChE3, a cDNA Encoding an Acetylcholinesterase of Boophilus microplus (Acari: Ixodidae)

Kevin B. Temeyer; John H. Pruett; Pia M. Untalan; Andrew C. Chen

doi:10.1603/0022-2585(2006)43[707:BEOBAC]2.0.CO;2

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Kevin B. Temeyer,^1,2 John H. Pruett,² Pia M. Untalan,² Andrew C. Chen²

¹Corresponding author, kevin.temeyer@ars.usda.gov
²Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA–ARS, 2700 Fredericksburg Road, Kerrville, TX 78028

Abstract

The complete cDNA sequence encoding a Boophilus microplus (Canestrini) (Acari: Ixodidae) acetylcholinesterase (AChE3) was expressed in the baculovirus system. The recombinant AChE3 protein (rBmAChE3) was secreted as a soluble form into the cell culture medium and was identified as a functional AChE by substrate specificity and by inhibition with the AChE-specific inhibitors eserine sulfate and BW284c51. Inhibition kinetics of rBmAChE3, in the presence of the organophosphate paraoxon, revealed sensitivity comparable with that of adult, organophosphate-susceptible neural AChE. To our knowledge, this is the first report of the cloning and successful expression of a functional ixodid AChE.

Citation Download Citation

Kevin B. Temeyer, John H. Pruett, Pia M. Untalan, and Andrew C. Chen "Baculovirus Expression of BmAChE3, a cDNA Encoding an Acetylcholinesterase of Boophilus microplus (Acari: Ixodidae)," Journal of Medical Entomology 43(4), 707-712, (1 July 2006). https://doi.org/10.1603/0022-2585(2006)43[707:BEOBAC]2.0.CO;2

Received: 12 January 2006; Accepted: 15 February 2006; Published: 1 July 2006